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ATCC
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Biomerix Corporation
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Corning Life Sciences
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Biomerix Corporation
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BioMimetic Therapeutics
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Stemline Therapeutics
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Multiplexion GmbH
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MBL International
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Endress+Hauser inc
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Pasteur Institute
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Image Search Results
Journal: iScience
Article Title: Phagocytosis by stroma confounds coculture studies
doi: 10.1016/j.isci.2021.103062
Figure Lengend Snippet: Phagocytosis is not restricted to NKTert but is also observed for MSCs and is not limited to dead cells of leukemic origin (A) NKTert also phagocytosed apoptotic cells of nonhaematopoietic origin. CellTracker-Green-labeled HBL-2, OCI-AML 2, HELA, or HEK-293T cells (green), treated with doxorubicine to induce apoptosis and cocultured with NKTert. Blank areas in staining of CellTracker-Blue-labeled NKTert (blue) were observed (examples indicated by arrows). These blank areas overlapped with CellTracker-Green (green)- and propidium-iodide (yellow)-stained HBL-2, OCI-AML 2, HELA, or HEK-293T cells. Some of these phagosomes were acidic, indicated by staining with lysosomal dye NIR (red). (B) CellTracker-Blue-labeled mesenchymal stromal cell (MSC; blue) phagocytosing dead CellTracker-Green-labeled CLL cells (green). White arrows indicate examples for phagosomes containing dead CLL cells. Red arrow indicates dead CLL cell in the process of being phagocytosed by NKTert. See also for 3D view. See also , , , and .
Article Snippet: This section describes the experiment shown in B. HEK-293T, HS-5, and
Techniques: Labeling, Staining
Journal: iScience
Article Title: Phagocytosis by stroma confounds coculture studies
doi: 10.1016/j.isci.2021.103062
Figure Lengend Snippet: Further characterization of phagocytic potential of bone marrow stromal cell lines (A) Flow cytometry of HS-5 and NKTert cells stained with antibodies against CD44, CD271, CD73, podoplanin (PDPN), CD26, CD90, CD105, CD31, and CD146. (B) Relative abundances of proteins in the lysosomal pathway for HS-5 and NKTert stromal cells, assessed by proteomics in four replicates. (C) Confocal microscopy of CellTracker Blue stained NKTert cocultured with 9- to 13-μM glass spheres for 16 h and stained with lysosomal dye NIR. See also , , and .
Article Snippet: This section describes the experiment shown in B. HEK-293T, HS-5, and
Techniques: Flow Cytometry, Staining, Confocal Microscopy
Journal: Frontiers in Bioengineering and Biotechnology
Article Title: Advances in ex vivo expansion of hematopoietic stem and progenitor cells for clinical applications
doi: 10.3389/fbioe.2024.1380950
Figure Lengend Snippet: Overview of the diversity of relevant strategies for ex vivo expansion of hematopoietic stem and progenitor cells.
Article Snippet: dECM matrices , UCB CD34 + were expanded on spin coated decellularized extracellular matrix from
Techniques: Ex Vivo, Clinical Proteomics, Cell Culture, Genetically Modified, Membrane, Construct